Figure 4
From: Identification of enzymes involved in SUMOylation in Trypanosoma brucei
Identification of the active cysteine residues of TbUba2 and TbUbc9. (a) In vitro SUMOylation of TbUbc9-HA using TbUba2-His or TbUba2C343A-His in the presence or absence of ATP. The thioester linkage between TbUba2 and TbSUMO was located at C343 residue of TbUba2. (b) in vitro SUMOylation of TbUbc9-HA, TbUbc9C113A-HA, TbUbc9C132A-HA and TbUbc9Δloop-HA with or without ATP. It indicates that the thioester linkage was formed between C132 residue of TbUbc9-HA and the GG motif of TbSUMO. The samples were fractionated by SDS-PAGE (12%) and analyzed by immune blotting using anti-HA antibody.
