Figure 5
From: Identification of enzymes involved in SUMOylation in Trypanosoma brucei
In vitro SUMOylation of TbCentrin3-HA and TbCentrin3 K0-HA in the presence or absence of ATP TbCentrin3 K0-HA was the mutant in which all the lysine residues were mutated to arginine except the deleted C-terminal lysine K164 and K165. The samples were fractionated by SDS-PAGE (12%) and analyzed by immune blotting using anti-HA antibody. SUMOylated Tbcentrin3-HA is indicated by asterisk (*).
