Figure 3
Identification and expression of Cbx3 gene of swamp eel.
a). Protein sequence alignments of Cbx3 from human, mouse, cattle, chicken, frog, salmon, zebrafish and swamp eel show an evolutionarily conservation. GenBank access number for swamp eel Cbx3 is KP054389. b) Phylogenetic tree depicts the Cbx family from human, mouse, cattle, chicken, frog, salmon, zebrafish and swamp eel. The numbers on the branches represent the boot-strap values from 100 replicates obtained using the maximum-likelihood method. The red color highlighs the Cbx3 cluster. c) Non-quantitative RT-PCR analysis in adult tissues from each sex of swamp eel. PCR product for each gene has been run under the same experimental conditions and same sizes of gel images have been used for all genes. d) Western blot assay shows Cbx3 expression in three gonad types of swamp eel. The protein gels have been run under the same experimental conditions and same sizes of gel images have been used for all proteins. The right panel shows the intensity values related to Actin. e) Immunohistochemistry assay shows that Cbx3 expression in gonads of swamp eel. Marked signals are observed in testis and then in ovotestis. The signals are hardly detected in ovary. O, ovary, Ode, developing ovary. f) Immunofluorescence analysis of Cbx3 in mouse testis using anti-Cbx3. Signals are observed in spermatogonia (sg), spermatocytes (sc) and sperm cells (s). The bar, 20 μm.
