Figure 2
Bioenergetic properties of M. smegmatis strains lacking the cytochrome bd or the cytochrome bc1 complex.
(A) Wild-type (WT) and mutant strains with knocked-out cytochrome bc1 complex (ΔqcrCAB :: hyg) or cytochrome bd (ΔcydA :: kan) were grown overnight, sub-cultured in fresh medium and incubated at 37 °C for 60 h. The optical density at 600 nm was measured in 20 min intervals. Data are representative of two independent experiments, each done in triplicate. (B) Cellular ATP levels in WT and mutant M. smegmatis as determined by the Luciferase method. (C) Oxygen consumption rates of inverted membrane vesicles from wild-type and mutant M. smegmatis strains using NADH as substrate. Data represent average plus standard error of the mean (SEM) for one experiment done threefold. One-way ANOVA was used for statistical analysis, NS: not significant (P value > 0.05), ** represent P value < 0.01.
