Figure 2 | Scientific Reports

Figure 2

From: Novel anti-thrombotic agent for modulation of protein disulfide isomerase family member ERp57 for prophylactic therapy

Figure 2

Target validation and the inhibition of PDI members by ADTM.

(a) Schematics of the target validation. (b) Proteins isolated from rat platelets were pretreated with DMSO or excess amount of untagged ADTM for 2 h, followed by treatment with BAA for another 2 h and compound-protein-complexes were enriched with NeutrAvidin-agarose resin. The precipitates were detected by immunoblot for indicated proteins of PDI family. (c) ADTM selectively inhibits ERp57. The recombinant PDI, ERp57 and ERp72 proteins were incubated with different concentrations of ADTM for 100 min and its catalytic activity was determined in insulin reduction assay.

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