Figure 2
Fungal compositions differ between colonic mucosa and feces and change during intestinal inflammation.
Colonic mucosa and feces were collected after the mice were sacrificed. (A) 16S rDNA (representing bacteria) and (B) 18S rDNA (representing fungi) levels in the colonic mucosa were analyzed by qRT-PCR and their copy numbers were calculated from the total amount of mucosal DNA (100 ng). (C) 16S rDNA and (D) 18S rDNA levels in the colonic fecal samples were analyzed by qRT-PCR and their copy numbers were calculated from the total amount of fecal DNA (100 ng). The values are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. (E) Phyla and (F) genera changes in 12 major fungal genera (average relative abundance ≥0.01 on average in colonic samples) for colonic mucosal and fecal samples after DSS treatment. Fungal compositions in the mouse diet were also detected. The Y-axis indicates the relative abundance of fungi at the phylum or genus level. Fungi found in the colon but not the food are marked with an octothorpe (#). (G) Heat maps showing the 27 core OTUs of fungal communities inferred from mucosal and fecal ITS1-2 sequences, with each mouse shown individually. The eight mice in each of the two treatment groups were each housed in two cages. The distribution of mice in cages is indicated at the bottom of the columns. The colored squares in each row indicate the relative abundance of the OTU among the 32 subjects. (n = 8/group). *P < 0.05, **P < 0.01, ***P < 0.001.
