Figure 2

iMSRC user’s interface (a), iMSRC applied to mosaic capturing (b) and detection of rare events (c).

iMSRC allows the automatic capturing of complex experimental designs by choosing previously saved files on extendable menus and tuning three simple image analysis parameters (a). Searching mosaic (b1 and c1), image-analysis-detected objects (b2 and c2) and final, merged-colour maximum projections (b3 and c3) are shown. Seventy-seven differently sized tissue pieces belonging to a Tissue Microarray were automatically captured overnight in (b). Circulating Tumour Cells (1:400) were automatically detected in (c). Insets show details. Text and icons show step specifics; see the Material and Methods section for further details. Scale bars (b1): 5 mm; (b3): 250 microns; (c1): 600 microns; and (c3): 12 microns.