Figure 7
Transplantation of the DLSs into the infrahepatic space of a live pig.
(A) The recipient’s renal vein (RV) and DLS’s portal vein (PV) (arrow) were used to perform end-to-end anastomosis between the recipient and the DLSs. The blood filled DLS immediately after reperfusion. (B) Blood perfused homogenously throughout the DLS within 5 min. The suprahepatic inferior vena cava (SHIVC) of DLS has been reconnected to the recipient’s splenic vein (SV) (arrow). (C) The perfused DLS after 1 h of perfusion. The blood flow out from SHIVC (arrow) of DLS. * EPA heparin-treated DLS. (D) H&E staining of the explanted DLSs shows vascular structures of control group DLSs are occluded by thrombosis (triangle) after 20 min of perfusion, whereas others are open with red blood cells (star) after 1 h of perfusion, from left to right: control, /h-LBL, /h-MPA and /h-EPA. 3 DLSs implantation for each group. Scale bars: 50 μm.
