Table 2 PCR primer sequences and product length.
Gene | Abbr. | Assession No. | Forward primers | Reverse primers | Size of product |
|---|---|---|---|---|---|
Porcine endogenous retrovirus | PERV | NC_003059 | 5′-CTACCCCGAGATTGAGGAGC-3′ | 5′-GGGGGATGGTTAGTTTTCCA-3′ | 297 bp |
PERV Polymerase | PERV Poly | EU_789636 | 5′-CTACAACCATTAGGAAAACTAAAAG-3′ | 5′-AACCAGGACTGTATATCTTGATCAG-3′ | 326 bp |
Swine leukocyte antigen DR alpha | SLA-DRA | NC_010443 | 5′-CGAGAAGAGGTGGCAAGACA-3′ | 5′-GTCCTGGAGGATTCCCTTGA-3′ | 343 bp |
Swine leukocyte antigen 2 | SLA-2 | NC_010449 | 5′-GTCACCTTGAGGTGCTGGG-3′ | 5′-TGGCAGGTGTAGCTCTGCTC-3′ | 185 bp |
Sus scrofa cytochrome b | SsCytB | NC_000845 | 5′-CATTGGAGTAGTCCTACTATTTACCG-3′ | 5′-GTAGGATTAGTATTATAAATAAGGCTCCT-3′ | 377 bp |
Porcine beta actin | β-actin | NC_010454 | 5′-TGTCATGGACTCTGGGGATG-3′ | 5′-GGGCAGCTCGTAGCTCTTCT-3′ | 277 bp |
