Figure 5
Profile of paracrine factors in the conditioned medium from iPSC-MSCs.
a) Functional classification of the top 20 factors preferentially over-presented in iPSC-MSC-CdM. Here the p-value, or called EASE score, is a modified one-tail Fisher exact probability value used for gene-enrichment analysis in DAVID system. Each gene group has been assigned several annotation terms and each gene group’ enrichment score stands for the geometric mean of negative denary logarithm of EASE scores of those terms involved in this group. Groups with enrichment score higher than 0.6 were shown in the figure (Enrichment score for group A: 1.25; B: 1.14; C: 0.81; D: 0.65; E: 0.61). b) Interactions and functional network identified by IPA. Genes in red indicate over-expressed genes found in iPSC-MSCs secretion compared with BM-MSC secretion. Deeper color means higher fold ratio for iPSC-MSCs- compared with BM-MSCs-secretion (i). MIF involved molecular and cellular functions identified by IPA. Ranged p-values are shown in negative denary logarithm form in y-axis (ii). GDF-15 involved molecular and cellular functions identified by IPA. Ranged p-values are shown in negative denary logarithm form in y-axis (iii). c) The concentration of GDF-15 among different groups was measured (i) (*p < 0.01 vs. BM-MSCs-CdM group, n = 3). The concentration among different groups was measured (iii) (*p < 0.01 vs. BM-MSCs-CdM group, n = 3).
