Figure 6 | Scientific Reports

Figure 6

From: Potent Paracrine Effects of human induced Pluripotent Stem Cell-derived Mesenchymal Stem Cells Attenuate Doxorubicin-induced Cardiomyopathy

Figure 6

Role of MIF and GDF-15 in iPSC-MSC-CdM-attenuated Dox-induced cardiotoxity.

a) Representative photographs showing ROS generation among Control, doxorubicin (Dox), iPSC-MSCs-CdM-control antibody treatment (iPSC-MSCs-CdM-ConAb), or MIF and/or GDF-15 depleted iPSC-MSCs-CdM treatment. b) Representative photographs showing the apoptosis of NRCMs among Control, doxorubicin (Dox), iPSC-MSCs-CdM -control antibody treatment (iPSC-MSCs-CdM-ConAb), or MIF and/or GDF-15 depleted iPSC-MSCs-CdM treatment. c) Quantitative measurement of immunofluorescent intensity of ROS among different experimental groups after normalized to control group (*p < 0.01, n = 3). d) The concentration of MDA among different groups was measured (*p < 0.01, n = 3). e) Quantitative measurement of apoptotic rate of NRCMs among different experimental groups. Quantitative measurement of ROS and apoptotic rate was counted from five viewing fields in each group and triple experiments were performed (*p < 0.01).

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