Table 2 The primer sequences of 27 differentially expressed genes and reference gene.
From: Large-scale identification of encystment-related proteins and genes in Pseudourostyla cristata
Gene number | Gene name | Primer sequence (5’-3’) | Annealing Temperature (0C) | PCR Product (bp) |
|---|---|---|---|---|
G1 | 17SrRNA | Sense:TGGTCGCAAGGCTGAAACTTA | 58 | 325 |
| Â | Â | Antisense:CAGGACATCTAAGGGCATCACA | Â | Â |
B18 | Ubiquitin family protein, mRNA (Tetrahymena thermophila) | Sense:GACCTCCTCTTCCTATGCTACC | 58 | 120 |
| Â | Â | Antisense:GTAATTCTGCTAAGCGATGGACAA | Â | Â |
B19 | ubiquitin-transferase, HECT-domain, mRNA (Tetrahymena thermophila) | Sense:CTGAAGAAGACATGAGTGAGGAAG | 58 | 156 |
| Â | Â | Antisense:CATCGTCCAAGTGGCTATCC | Â | Â |
B28 | ABC (ATP-binding cassette) transporter family protein, mRNA (Tetrahymena thermophila) | Sense:TCAGGATGTGGCAAATCTACGA | 58 | 200 |
| Â | Â | Antisense:GCATCTGGCTTGGCATATCTTAG | Â | Â |
G2 | Calmodulin(Stylonychialemnae) | Sense:GCAGACGGTAACGGAACCATT | 56 | 240 |
| Â | Â | Antisense:CCATCATCATTCTGACGAATTC | Â | Â |
G3 | HSP70 (hsp70) gene, complete cds; macronuclear (Pseudourostyla cristata) | Sense:TCTCCTCCTAAGATACCTCCTTGA | 60 | 222 |
| Â | Â | Antisense:GACAATTACAAGAGCCAGGTTTG | Â | Â |
G4 | Cathepsin B (Naegleria gruberi) | Sense:GTTATAGGATTATTGGGATCTCATG | 56 | 199 |
| Â | Â | Antisense:AACCTTGTTTACCTTGCCGTTG | Â | Â |
G5 | strain BA PAP2 superfamily phosphatase gene, complete cds; macronuclear (Sterkiella histriomuscorum) | Sense:TGGGCACACTTTTCAAGCTAT | 56 | 160 |
| Â | Â | Antisense:ACACCCAAAAGCCATCCATA | Â | Â |
G8 | Actin I (Urostyla grandis) | Sense:GTCAAGGCAGAGCAAGGATG | 60 | 330 |
| Â | Â | Antisense:CACAGCACACGCAATGCCTTA | Â | Â |
G9 | Calcium transporting ATPase (Paramecium falciparum) | Sense:AGAAGACTGGGATGGAAACACT | 60 | 330 |
| Â | Â | Antisense:CCAAAGGTCATTGCCTGAACTAA | Â | Â |
G10 | Voltage-dependent Calcium Channel (Mus musculus) | Sense:TCATCGGCATCAATTTAACCAA | 55 | 240 |
| Â | Â | Antisense:TTTCCAACAGGAATCCCAAGAT | Â | Â |
G11 | Ser/Thr specific protein phosphatas (Gallus gallus) | Sense:GGAATGCACAAAGGGAAAGTGTT | 60 | 399 |
| Â | Â | Antisense:AGTGGGTAACTCTGAGGATAAGT | Â | Â |
G12 | Cytochrome c oxigdase subunit I (Sterkiella histriomuscorum) | Sense:GCCATCAATCCGGCAACACTTA | 60 | 237 |
| Â | Â | Antisense:ATGCTGGCAGGAGCGGCTATGT | Â | Â |
G14 | 16S ribosomal RNA gene, complete sequence (Pseudokeronopsis rubra) | Sense:TCATAACAACTGATCGAATCGC | 58 | 174 |
| Â | Â | Antisense:GCCTTCCTTAGATGTGGTAGCC | Â | Â |
G15 | voucher QDHXZ2007102801 alpha-tubulin gene, partial cds (Pseudourostyla cristata) | Sense:AGAACCCACTGTTATTGATGAA | 58 | 117 |
| Â | Â | Antisense:GTGTAATGACCTCTGGCGAAG | Â | Â |
G16 | small subunit ribosomal RNA gene, partial sequence (Pseudourostyla cristata) | Sense:TGGAGTGATTTGTCTGGTTAAT | 58 | 196 |
| Â | Â | Antisense:TCGCTGTATGCGTCAGTGTAG | Â | Â |
G17 | cytochrome b (CYTB) gene, complete cds; mitochondrial (Pseudourostyla cristata) | Sense:GAAGGGTTAATGTGGGTTGGT | 58 | 101 |
| Â | Â | Antisense:TAGTTGGAATGTGTTTATTGTGAGT | Â | Â |
G19 | partial 18S rRNA gene (Pseudourostyla franzi) | Sense:TCAGCTTTCGATGGTAGTGTAT | 58 | 176 |
| Â | Â | Antisense:GCTCAGTCCGTTATTTCTTGTC | Â | Â |
M3 | IP1 phosphoserine aminotransferase, putative (EIN_083770)mRNA, complete cds (Entamoeba invadens) | Sense:GGAGCAAGACTGGAAAGATGAAGA | 58 | 158 |
| Â | Â | Antisense:AGTAGCACCATTGGGCCATAATTAA | Â | Â |
M4 | minichromosome Ca2+-ATPase (PMCA) gene, complete cds (Sterkiella histriomuscorum) | Sense:TATGTTTATCGTTTTCCTCGGTGG | 58 | 170 |
| Â | Â | Antisense:TGGTATCATTTCTGGAGTATGGCTT | Â | Â |
M9 | ATP synthase F1, alpha subunit family protein, mRNA (Tetrahymena thermophila) | Sense:GTCGGTGTTGTCGTTTTGGGTAAT | 58 | 176 |
| Â | Â | Antisense:ACTCTGGCTCTTTGGGTGGTCTTA | Â | Â |
M14 | Dynein heavy chain family protein, mRNA (Tetrahymena thermophila) | Sense:TACATGCCTGAACCTGACTATCCAG | 58 | 122 |
| Â | Â | Antisense:CAAGCCACTACTTCGCTCATTAACT | Â | Â |
M15 | 14-3-3 protein, mRNA (Tetrahymena thermophila) | Sense:ACCACTCACCCCATCAGATTAGG | 58 | 108 |
| Â | Â | Antisense:ATCGAAAGCAGTCTTAGCCAAGG | Â | Â |
Z5 | 26S proteasome non-ATPase regulatory subunit Nin1/mts3 family protein, mRNA (Tetrahymena thermophila) | Sense:GGAGTTAGTTCCAGTTGAAGACT | Â | Â |
| Â | Â | Antisense:CGAAGTGAGCAAGAGGAGAC | 60 | 134 |
Z13 | Homo sapiens dynein, axonemal, heavy chain 12(DNAH12), transcript variant 2, mRNA | Sense:TGAGTTTGGTGGAACGAATAG | 59 | 147 |
| Â | Â | Antisense:CTGCTGCCTTCAGTGTATC | Â | Â |
Z22 | outer membrane protein, putative, mRNA (Tetrahymena thermophile) | Sense:CTAGTGCAACGCTAACTTCTATGGC | 59 | 189 |
| Â | Â | Antisense:CTTAGTTCTTCGTCGGAGGTCAAAC | Â | Â |
Z23 | clathrin, light chain A (CLTA), mRNA (Bos taurus) | Sense:GACGAATGGAGACTACTACC | 58 | 164 |
| Â | Â | Antisense:CTTCCTGCTTCCGAGAAT | Â | Â |
Z24 | Clathrin adaptor complex small chain family protein, mRNA (Tetrahymena thermophila) | Sense:TAGACTCGCAAAATGGTATGTAGAC | 56 | 170 |
| Â | Â | Antisense:CGCAAATTGAAAAGAACAGACC | Â | Â |
