Figure 4
(a) Confocal image of MIA PaCa-2 cells following a 30 min exposure to MM41. The fluorescence of the compound is seen concentrated in the cell nuclei. (b) Images (x 40) of tumour tissue from control and MM41-treated MIA PaCa-2 xenografts, showing DAPI-stained and MM41 fluorescent images, together with merged images showing superposition of DAPI and MM41 staining. The images are of sections close to the tumour surface. (c) Images (x 20) showing DAPI and MM41 staining, together with the merged image, for sections taken from the centre of a treated tumour. (d) (LHS) IVIS whole-body fluorescence image, taken at the MM41 emission wavelength, showing that MM41 is concentrated in the tumour, which is on the flank of the animal. A substantial amount of MM41 is shown to be retained at the site of administration. An image of the extracted tumour is also shown.
