Figure 3 | Scientific Reports

Figure 3

From: Cortical actin networks induce spatio-temporal confinement of phospholipids in the plasma membrane – a minimally invasive investigation by STED-FCS

Figure 3

Experimental observation of lipid compartmentalised diffusion by STED-FCS.

Dapp(d) dependencies (blue) for DPPE-Atto647N diffusion in NRK (a) and IA32 cells (b). Clear compartmentalised diffusion patterns are observed. **P < 0.01 (unpaired t test). Error bars are s.e.m. In a, n = 32 cells; in b, n = 33 cells. In a and b, r = 10 (n stands for the number of cells, from r samples). Fitting of the experimental data using Monte-Carlo simulations (orange dotted lines) resulted for NRK cells, Dfree = 0.8 ( ±0.03) μm2/s, Phop = 0.1 ( ±0.01) and L = 80 ( ±8) nm and for IA32 cells, Dfree = 0.8 ( ±0.02) μm2/s, Phop = 0.1 ( ±0.01) and L = 150 ( ±12) nm. Insets: Representative Voronoi lattices (red) relative to the correspondent compartment sizes as well as simulated diffusion trajectories (blue) correspondent to the fitted parameters. Scale bars: 250 nm.

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