Figure 4 | Scientific Reports

Figure 4

From: Cortical actin networks induce spatio-temporal confinement of phospholipids in the plasma membrane – a minimally invasive investigation by STED-FCS

Figure 4

Investigation of molecular mechanisms underlying compartmentalised lipid diffusion via STED-FCS: Pharmacological treatments.

Dapp(d) dependencies: (a,b) Cytoskeleton depletion in NRK (a) and IA32 (b) cells, respectively: treatment with Latrunculin B and CK-666 as labelled, (c,d) Cholesterol depletion and myosin II inhibition in NRK (c) and IA32 (d) cells, respectively: treatment with Cholesterol Oxidase and Blebbistatin as labelled. (e,f) Summary of NRK and IA32 data, respectively, showing values of Dapp for confocal (d = 240 nm, filled columns) and STED recordings (d ~ 40 nm, open columns) – the increase in Dapp from 240 to 40 nm indicates the extent of compartmentalised diffusion. Error bars are s.e.m. Symbols on top of the columns represent results of the statistical test (**P < 0.01, *P < 0.05, NS not significant; two-tailed unpaired t test): for d ~ 40 nm comparison with the value representing d = 240 nm in the same experiment and for d = 240 nm comparison with the respective value for d = 240 nm in the control (untreated) experiment. n stands for the number of cells, from r samples.

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