Figure 5 | Scientific Reports

Figure 5

From: Uric Acid Produces an Inflammatory Response through Activation of NF-κB in the Hypothalamus: Implications for the Pathogenesis of Metabolic Disorders

Figure 5

Inhibition of NF-Κb protects mice against the hypothalamic inflammation induced by UA.

(A–D) Expression of Nfkbia (A), Il6 (B), Tnfa (C) and Il1b (D) were determined by qRT-PCR in the hypothalamus of the mice that were icv injected with normal saline (N.S.), UA (3000 ng/ml, 2 μl) or allantoin (3000 ng/ml, 2 μl) for 2 weeks (n = 6 mice per group). (E–H) Expression of Nfkbia (E), Il6 (F), Tnfa (G) and Il1b (H) were determined by qRT-PCR in the hypothalamus of the mice that were icv injected with normal saline (N.S.) or UA (600 ng/ml or 3000 ng/ml, 2 μl), with or without icv injection of BAY11-7085 (n = 6 mice per group). All displayed values are the mean ± SEM. *P < 0.05; **P < 0.01 versus saline control; #P < 0.05; ##P < 0.01 versus without BAY11-7085 administration.

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