Figure 2
The overexpression of A3G promotes the xenograft tumor formation rate but inhibits tumor growth in nude mice.
(A) Establishment of stable A3G expressing BxPC3 cells. BxPC3 Cells with stable A3G expression were established by recombinant lentivirus infection (Scale bar 100 μm). (B,C) The detection of A3G in stably expressing BxPC3 cells. Real-time qPCR and western blots revealed that A3G expression was significantly higher in stable A3G-expressing BxPC3 cells than in control cells (**P < 0.01, Student’s t-test). (D) Determination of cell proliferation. Cell proliferation was quantified by MTS assay. The data are represented as the means ± SEM of triplicate experiments (**P < 0.01, Student’s t-test). (E) Overexpression of A3G promotes the tumor formation rate. A3G-expressing BxPC3 cells and control cells were injected subcutaneously into nude Balb/c mice and mice were followed for 4 weeks. The tumor formation rate was significantly higher in the A3G-expressing group than in controls (*P < 0.05, **P < 0.01, χ2 test).
