Figure 1

The visualization of the vascular wall using adaptive optics scanning laser ophthalmoscopy.

(a) The fundus photograph of normal left eye. (b) The adaptive optics images in the area outlined in white on panel (a). The arterial wall was successfully visualized along the artery. However, the venous wall could not be visualized. (c) Magnified images outlined in white on panel (b). The interval between the tips of the 2 red arrowheads represents the arterial wall. Scale bar, 100 μm. (d) Magnified images outlined in red on panel (b). Scale bar, 100 μm. (e) The same magnified image as in panel (d) with the result from semi-automatic segmentation of vessel border lines. Retinal arterial wall borders were detected along the vessel’s running direction. After control points for the retinal arterial axes were set manually (blue line), vascular wall border detection was processed automatically Green and pink line represent outer wall borders. Yellow and orange line represent inner wall borders. Scale bar, 100 μm.