Figure 2
From: Characterization of RNA silencing components in the plant pathogenic fungus Fusarium graminearum
Simultaneous silencing of multiple genes in F. graminearum via expression of hpRNA with chimeric inverted repeats.
(a) Schematic representation of the chimeric inverted-repeat fragment construction. The target fragment of FgCYP51A, FgPKS12, and the DON biosynthesis gene FgTRI6 was constructed using the joint PCR strategy and then inserted into the multiple cloning site of pSilent-1. (b) Phenotypic analyses of the silencing transformant. The silencing transformant CPT-S2 showed increased susceptibility to triadimefon (5 μg mL−1; left top), displayed white colony (left bottom) and produced drmatically less DON than the wild type HN9-1 (right panel).
