Figure 4
From: Characterization of RNA silencing components in the plant pathogenic fungus Fusarium graminearum
Relative mRNA expression patterns of argonaute and dicer genes.
(a) The relative mRNA expression level of argonaute or dicer genes in conidia represents the relative amount of each gene mRNA in the mycelia determined by quantitative RT-PCR. Line bars indicate standard errors from three repeated experiments. (b) Localization of the FgDicer2-GFP fusion protein in mycelia and conidia. Fresh mycelia and conidia were collected and visualized with a Zeiss LSM780 confocal microscope for GFP signals. To observe nuclei, fresh mycelia or conidia were washed with sterilized water and stained with 10 μg mL−1 4′6-diamidino-2-phenylindole (DAPI, Sigma).
