Figure 4
From: Subcellular and Dynamic Coordination between Src Activity and Cell Protrusion in Microenvironment
Quantify the dynamic Src activity and membrane protrusion at the edge of a polarized cell.
(A) The spatiotemporal map of ECFP/FRET ratio at cell edge; (B) The time courses of Src FRET ratio in a representative sampling window at the protrusive edge (P) and non-protrusive edge (NP); (C) The line scans of Src activity along the cell edge before and after removing the gel membrane; (D) The spatiotemporal map of cell boundary translocation; (E) The time courses of edge translocation at the protrusive (P) and non-protrusive (NP) edges; (F) The line scans of translocation at the cell edge before and after removing the gel membrane; (G) The temporal cross-correlation (CC) map between Src activity and cell edge translocation; (H) temporal CC between Src activity and boundary translocation in two representative sampling windows (P-region and NP-region); (I) spatial CC between Src activity and boundary translocation; (J) spatial CC between Src activity and boundary translocation before and after removing the PDMS membrane.
