Figure 5
From: Subcellular and Dynamic Coordination between Src Activity and Cell Protrusion in Microenvironment
The correlation of Src activity and boundary translocation at the edge of polarized cells.
The time courses of normalized ECFP/FRET emission ratio sampled at (A) the protrusive edge, or (B) non-protrusive edge, of multiple cells; the time courses of translocation sampled at (C) the protrusive edge, or (D) non-protrusive edge, of multiple cells. (E) The maximal ECFP/FRET ratio at the protrusive (P) and non-protrusive (NP) edges are compared before and after peeling-off the PDMS gel membrane; (F) The translocation of cell boundary at the protrusive (P) and non-protrusive (NP) edges are compared before and after peeling-off the PDMS gel membrane. The temporal CC at (G) the protrusive edge, or (H) non-protrusive edge, of multiple cells. Grey dots-data; black solid lines with dots-the average curve. *indicates significant difference by t-test, two tailed, number of cells = 3, number of samples = 40, p-value < 0.05.
