Figure 1

Detection of antagonistic action against Xanthomonas oryzae pv. oryzae

(A) and Xanthomonas oryzae pv. oryzicola (B) by paper-disc agar diffusion assay. Bactericidal activity was tested as described in Methods. Control (Landy medium), FZB42 (wild type, producer of lipopeptides, polyketides and bacilysin), CH3 (Δsfp::Em^r, deficient in lipopeptide and polyketide synthesis), AK1 (ΔbmyA::Em^r, deficient in bacillomycin D synthesis), AK2 (ΔfenA::Cm^r, deficient in fengycin synthesis), CH1 (ΔsrfA::Em^r, deficient in surfactin synthesis), CH6 (Δbae::Cm^r, no synthesis of bacillaene), CH7 (Δmln::Cm^r, no synthesis of macrolactin), CH8 (Δdfn::Em^r, no synthesis of difficidin), RS6 (Δsfp::Em^r Δbac::Cm^r, no lipopeptides, polyketides or bacilysin) and RS2 (Δbac::Cm^r Δdfn::Em^r, deficient in bacilysin and difficidin). The diameters of inhibition zones (mm) included the paper disk diameter (5 mm). Data are expressed as means ± standard deviation (SD). − indicates no inhibitory activity. **indicates an extremely significant difference compared with FZB42 (P < 0.01).