Figure 4
From: The Salmonella effector protein SifA plays a dual role in virulence
SifA C-term function in infected cells.
HeLa cells (A,B) and RAW 264.7 mouse macrophages (C) were infected with wild type (WT) S. Typhimurium, a ∆sifA mutant or a sifAL130D strain. (A,B) The SifA C-term increases the presence of LAMP1 on the SCV. (A) HeLa cells were infected for 14 h, immunostained and imaged for bacteria (green), LAMP1 (red) and DNA (light blue) using a confocal microscope. Magnified insets showing single labelling for GFP (left) and LAMP1 (right) are presented below each image. Bar, 20 μm or 10 μm for the magnified insets. (B) SCVs were scored for the presence or absence of LAMP1. Percentages were normalized to that of the wild type strain. Results are the means ± SD of three independent experiments. (C) The SifA C-term supports Salmonella intracellular replication. RAW 264.7 mouse macrophages were infected with different Salmonella strains and lysed at 2 or 16 h post-infection for enumeration of intracellular bacteria. The values shown represent the fold increase calculated as a ratio of the intracellular bacteria between 16 and 2 h and normalized to that of the ∆sifA strain. Values are means ± SD of four independent experiments. Unpaired t-tests were used to compare two values. P values: **P < 0.01.
