Figure 3 | Scientific Reports

Figure 3

From: Identification of the interaction of VP1 with GM130 which may implicate in the pathogenesis of CVB3-induced acute pancreatitis

Figure 3

GM130 specifically interacts with VP1 of CVB3.

(a) HeLa cells were transiently transfected with His-tagged VP-1, VP-2, 2C or 3D plasmids. The cell lysates were harvested and immunoprecipitated with monoclonal anti-GM130 or anti-His antibodies. Immune complexes and the input were analyzed by western blot analysis using anti-GM130 antibody or anti-His antibody. (b) Yeast reporter strain AH109 was cotransformed with pGADT7-GM130 and the different viral proteins in pGBKT7 as indicated. pGBKT7-53 served as a positive control, whereas pGBKT7 served as a negative control. The transformed cells were analyzed for α-Galactosidase staining.

Back to article page