Figure 5

CVB3 infection dissociates GM130-GRASP65 complexes in HeLa cells.
(a) Cellular lysates of HeLa cells with or without CVB3 infection (MOI of 5) were immunoprecipitated with antibodies against GM130 or GRASP65. Immunoprecipitates and cellular lysates were analyzed using antibodies against GM130, VP1 or GRASP65 by western blot. (b) Immunofluorescent staining with antibodies against GM130 or GRASP65 at indicated time points in HeLa cells infected with CVB3 (MOI of 5). Scale bars, 10 μm.