Figure 8
GM130 expression may contribute to the tissue specificity of CVB3.
(a) GM130 expression in the pancreas from the mice at the age of 4–6 weeks that were infected with CVB3. Tissue proteins were collected at the indicated time points and analyzed by Western blot with anti-GM130, anti-VP1 and anti-α-Tubulin. (b) GM130 expression in the hearts from the mice at the age of 4–6 weeks that were infected with CVB3. Tissue proteins were collected at the indicated time points and analyzed by Western blot with anti-GM130, anti-VP1 and anti-α-Tubulin. (c) GM130 expression in the pancreas and hearts from the mice at the age of 2 days or 4–6 weeks was examined by Western blot. Tubulin was probed to serve as a loading control. (d) Generation of siRNA-mediated knockdown stable cell lines. HeLa cells were transfected with the plasmids for si-Control or si-GM130. Positive clones were selected by incubation with G418. The knockdown stable cell lines were analyzed by western blot analysis with anti-GM130. (e) CVB3 replication in GM130-knocking down stable cell lines.
