Figure 2
Electrophysiological characterization of KCNQ1/KCNE1 and Iks in response to different voltages and toxin concentrations.
(A) Guinea pig Iks elicited by depolarizing pulses from −30 mV to +70 mV and subsequent deactivating tails at –30 mV in the absence (top) and presence (bottom) of 1 μM SSD609. (B) Dose–response Iks channel conductance measurements at different SSD609 concentrations. IC50 was calculated to be 209.3 ± 60.9 nM (n = 5). (C) G/V curves of Iks in the absence (full line, V50 = 50 mV, n = 7) and presence (dotted line, V50 = 56 mV, n = 4) of 1 μM SSD609. (D) KCNQ1/KCNE1 current elicited by depolarizing pulses from −80 mV to +60 mV in the absence (top) and presence (bottom) of 1 μM SSD609. (E) Dose–response between KCNQ1/KCNE1 channel conductance and different SSD609 concentrations. IC50 was calculated to be 652.7 ± 260.6 nM (n = 5). (F) G/V curve of KCNQ1/KCNE1 in the absence (full line, V50 = 40 mV, n = 5) and presence of 1 μM SSD609 (dotted line, V50 = 46 mV, n = 4).
