Table 1 ccMA production by engineered strains with different constitutive promoters or an inducible promoter*.

From: Engineering catechol 1, 2-dioxygenase by design for improving the performance of the cis, cis-muconic acid synthetic pathway in Escherichia coli

Strains

ccMA (g/L)

Catechol (g/L)

Yield** (%)

E. coli WZK

0.34 ± 0.05

0.92 ± 0.08

3.9 ± 0.6

E. coli WZC

0.43 ± 0.02

0.85 ± 0.07

5.3 ± 0.3

E. coli WZT

0.71 ± 0.03

0.45 ± 0.03

9.3 ± 0.3

E. coli WZPL25

1.21 ± 0.07

0.07 ± 0.02

22.2 ± 0.5

E coli WZI*

1.15 ± 0.12

0.09 ± 0.02

20.1 ± 0.3

  1. *his strain was a control. During fermentation, 1 mM IPTG was added to the fermentation medium to induce the expression of catechol 1,2-dioxygenase. Errors (standard deviations) were calculated based on at least three independent experiments.
  2. **Yield: produced ccMA against consumed glucose.
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