Figure 5
Osteoclast differentiation was reduced in Col2-Opg mice.
(A) Primary chondrocytes derived from WT and Col2-Opg mice were co-cultured with WT spleen cells in the presence of 10−8 M 1,25-(OH)2 D3 (blue arrows: multinuclear osteoclasts). (B,C) Osteoclast formation was also studied in bone marrow cells derived from Col2a1-Opg transgenic mice in the presence of 50 ng/ml sRANKL and 10 ng/ml M-CSF. (D) Flow cytometry analysis was performed using monoclonal antibodies against RANK and CD11b (n = 3 per group). Data are presented as means ± SD.
