Figure 7
Osteoblast and adipocyte differentiation was slightly altered in Col2-Opg mice.
(A,B) ALP and Alizarin red O staining was performed using BMSCs from WT and Col2-Opg mice. (C) Real-time PCR was performed to examine expression of osteoblast marker genes. (D) Oil red O staining was performed to determine adipocyte differentiation from BMSCs derived from WT and Col2-Opg mice (green arrows: Oil red O staining-positive cells). (E) Real-time PCR was performed to examine expression of adipocyte marker genes. Data are presented as means ± SD (n = 4 per group).
