Figure 2
Phylogenetic analyses of the ABCH genes in P. xylostella(A) and the ABCH1 genes in diverse species (B).
(A) An unrooted neighbor-joining (NJ) tree was constructed on the basis of analyzing the amino acid sequences of the nucleotide binding domain (NBD) to show the phylogenetic relationships of ABCH subfamily genes with ABCA and ABCG subfamilies. Intriguingly, a previously undiscovered ABCG gene (Pxbrown) in the Diamondback moth Genome Database (DBM-DB, http://iae.fafu.edu.cn/DBM/index.php)45 has been found in the recent P. xylostella genome well re-annotated by NCBI (GenBank accession no. XM_011555870) and has been shown in the tree. (B) An unrooted neighbor-joining (NJ) tree was constructed on the basis of analyzing the full-length amino acid sequences to show the phylogenetic relationships of ABCH1 genes from insect and non-insect species. Multiple amino acid sequence alignments for both of the phylogenetic analyses were generated by ClustalW and the trees were subsequently created with MEGA 6.0. Bootstrap values expressed as percentages of 1000 replications are shown at branch points. GenBank accession numbers or Gene ID from other genome database sources are displayed within the tree and are indicated in parentheses. The scale bar indicates distance in number of substitutions per site. Abbreviations: 1. Hymenoptera (Amel, Apis mellifera; Nv, Nasonia vitripennis; Ae, Acromyrmex echinatior); 2. Coleoptera (Tc, Tribolium castaneum); 3. Hemiptera (Ap, Acyrthosiphon pisum); 4. Anoplura (Phc, Pediculus humanus corporis); 5. Diptera (Dm, Drosophila melanogaster; Md, Musca domestica; Aa, Aedes aegypti; Ag, Anopheles gambiae; Cq, Culex quinquefasciatus); 6. Lepidoptera (Bm, Bombyx mori; Dpl, Danaus plexippus; Px, Plutella xylostella); 7. Non-insect Arthropods (Tu, Tetranychus urticae; Dp, Daphnia pulex). 8. Non-insect Metazoa (Dr, Danio rerio; Amex, Astyanax mexicanus; Pn, Pundamilia nyererei; Mz, Maylandia zebra; Spa, Stegastes partitus; Pf, Poecilia formosa; Tr, Takifugu rubripes; Pr, Poecilia reticulata; Ol, Oryzias latipes; Cs, Cynoglossus semilaevis; Nb, Neolamprologus brichardi; Hb, Haplochromis burtoni; Lo, Lepisosteus oculatus; On, Oreochromis niloticus; Spu, Strongylocentrotus purpuratus). 9. Non-insect Prozotoa (Dd, Dictyostelium discoideum).
