Table 1 Effect of silencing of the PxABCH1 gene on some biological parameters of the surviving larvae in P. xylostella strain DBM1Ac-S and NIL-R.

Methods	Strain	Treatment	Pupation rate (%)	Pupal weight (mg)	Eclosion rate (%)
Microinjection	DBM1Ac-S	Buffer	100.00 ± 0.00^a	5.12 ± 0.11^a	88.89 ± 11.11^a
		dsEGFP	100.00 ± 0.00^a	5.03 ± 0.14^a	100.00 ± 0.00^a
		dsPxABCH1	55.56 ± 11.11^b	3.70 ± 0.20^b	0.00 ± 0.00^b
	NIL-R	Buffer	100.00 ± 0.00^a	5.06 ± 0.11^a	100.00 ± 0.00^a
		dsEGFP	100.00 ± 0.00^a	5.10 ± 0.05^a	88.89 ± 11.11^a
		dsPxABCH1	44.44 ± 11.11^b	3.82 ± 0.09^b	0.00 ± 0.00^b
Oral delivery	DBM1Ac-S	Buffer	100.00 ± 0.00^a	5.08 ± 0.15^a	100.00 ± 0.00^a
		dsEGFP	100.00 ± 0.00^a	5.12 ± 0.09^a	100.00 ± 0.00^a
		dsPxABCH1	44.44 ± 11.11^b	3.82 ± 0.12^b	0.00 ± 0.00^b
	NIL-R	Buffer	100.00 ± 0.00^a	5.04 ± 0.12^a	88.89 ± 11.11^a
		dsEGFP	100.00 ± 0.00^a	5.07 ± 0.10^a	100.00 ± 0.00^a
		dsPxABCH1	55.56 ± 11.11^b	3.75 ± 0.07^b	0.00 ± 0.00^b

Values shown in the table are means and standard errors, and values with different letters in a column for each strain indicated significant differences as determined by Holm-Sidak’s test (P < 0.05; n = 3).

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