Figure 2
From: Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining
Characteristics of the PLN VH:VL repertoire in CD138+ antibody secreting cells.
(a) Polarization of VH:VL repertoire after immunization with EBOV VLPs. The frequency of each unique CDRH3:CDRL3 antibody clonotype is shown as a percentage of total sequencing read counts. CDRH3 sequencing reads having at least 96% identity at the nucleotide level were clustered and compiled, then analyzed for corresponding CDRL3 per pair. Sequences identified in <2 reads were excluded to minimize sequencing error33. Inset: frequency of the ten most frequently observed CDRH3:CDRL3 antibody clonotypes from each mouse. (b,c) VH:VL gene family usage of unique CDRH3:CDRL3 clonotypes in mouse ZM1 and ZM2, respectively.
