Table 2 SPR Binding kinetics and equilibrium dissociation constants (KD) towards uncleaved EBOV GP.

From: Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining

Antibody ID

kon (M−1s−1)

koff (s−1)

K D (nM)

ZM1.1

(3.12 ± 0.55) × 104

(1.16 ± 0.07) × 10−3

37.7 ± 0.55

ZM1.2

(1.17 ± 0.41) × 104

(1.71 ± 0.12) × 10−3

156 ± 47.4

ZM1.3

(3.12 ± 0.53) × 104

(2.39 ± 0.62) × 10−4

7.71 ± 1.77

ZM1.6

(3.95 ± 0.7) × 103

(2.46 ± 0.12) × 10−3

635 ± 93.8

ZM2.1

(1.47 ± 0.23) × 104

(2.97 ± 0.07) × 10−4

20.5 ± 3.44

  1. Experiments were performed in three technical replicates and data were fit to a 1:1 Langmuir binding model.
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