Table 1 Percentage of identity of LGTV primers and probe relative to ORF of the E gene of LGTV and closely related viruses.
From: Detection of Langat virus by TaqMan real-time one-step qRT-PCR method
Virus | Target gene | Position 1607–1625 relative to Langat ORF (forward primer) 5′-TGGCAGGTGCATCGTGACT-3′ | Position 1652–1671 relative to Langat ORF (reverse primer) 5′-GCCTCAGCTCCATCATGCTT-3′ | Position 1628–1646 relative to Langat ORF (probe) 5′-TTTAATGATCTGGCCCTCC-3′ | |||
|---|---|---|---|---|---|---|---|
LGTV | E | TGGCAGGTGCATCGTGACT | 100% identical | AAGCATGATGGAGCTGAGGC | 100% identical | TTTAATGATCTGGCCCTCC | 100% identical |
TBEV | E | TGGCAGGTCCATAGGGACT | 84.21% identical | AAACATGAGGGAGCGCAAAA | 65% identical | TTCAATGATCTGGCTCTGC | 84.21% identical |
Louping ill | E | TGGCAGGTCCACCGAGACT | 84.21% identical | AAGCATGATGGAAACCCACA | 60% identical | TTTAATGACCTGGCTCTCC | 89.47% identical |
OHFV | E | TGGCAGGTTCACAGAGATT | 73.68% identical | AAACATGAGGGAATGGTGGG | 65% identical | TTTAACGATCTAGCTCTGC | 78.95% identical |
ALKV | E | TGGCAGGTGCACCGAGACT | 89.47% identical | CGACACGAGGGCGCCCATGA | 50% identical | TTTGAAGACCTCTCCTTGC | 63.16% identical |
KFDV | E | TGGCAGGTACACCGAGACT | 84.21% identical | CGACACGGGGGTGCCCAGGA | 50% identical | TTTGAGGACCTCTCCTTGC | 63.16% identical |
POWV | E | TGGCAAGTGCACCGTGACT | 89.47% identical | AAACACAAGGACAACCAAGA | 40% identical | TTTGAGGACTTGGCGCTGC | 68.42% identical |
