Figure 5 | Scientific Reports

Figure 5

From: Punicalagin, an active component in pomegranate, ameliorates cardiac mitochondrial impairment in obese rats via AMPK activation

Figure 5

PU induces mitochondrial biogenesis via AMPK pathway activation.

Neonatal cardiomyocytes were treated with either PE at 100 and 250 μg/mL or PU at 100 μg/mL for 24 h, protein levels of PGC-1α, complex I and II were analyzed by western blot (A) arbitrary unit was analyzed for PGC-1α (B) complex I (C) and complex II (D) Cells were then treated with PE or PU for 24 h either in the presence or absence of compound C, protein levels of p-AMPK, AMPK, PGC-1α and complex I were analyzed by western blot (E) arbitrary unit was analyzed for PGC-1α (F) and complex I (G) mitochondrial DNA copy number was analyzed by real-time PCR (H). The values are presented as the means ± S.E.M. (n = 4); *p < 0.05, **p < 0.01.

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