Figure 5
Expression of ompS38 is under the control of Crp.
(A) Map of the S. oneidensis ompS38 promoter region. The Crp-binding site was in bold and underlined. The locations of transcription initiation sites are in box, which were determined by 5′-RACE described in the text (next section). (B) Activities of PompS38 in strains indicated. The promoter was placed in front of E. coli lacZ gene and integrated into the chromosome of the strains indicated. Cells of mid-log phase were assayed by measuring β-galactosidase activity. (C) Production of OmpS38 in indicated strains. The lanes shown here were from the same gel, which was given in Fig. S3A. (D) EMSA assay was performed in the presence of 0, 0.5, or 2 μM Crp, 10 μM cAMP and 2 nM radiolabeled promoter DNA. 0.2 μg/μl poly(dI·dC) was used in all binding reactions to block nonspecific interactions. Promoter region of gyrB was used as negative control. In this figure, experiments were conducted independently at least three times and error bars represent S.D. or the representative was presented.
