Table 1 Strains and plasmids used in this study.
Strain or plasmid | Description | Reference or source |
|---|---|---|
E. coli strains | ||
DH5α | Host for cloning | Lab stock |
WM3064 | ΔdapA, donor strain for conjugation | W. Metcalf, UIUC |
XL1-Blue MRF’Kan | Recipient strain for one-hybrid system | Stratagene |
S. oneidensis strains | ||
MR-1 | Wild type | ATCC 700550 |
HG0624 | Δcrp derived from MR-1 | (35) |
HG1215 | ΔSO1215 derived from MR-1 | This study |
HG1821 | ΔSO1821 derived from MR-1 | This study |
HG1937 | ∆fur derived from MR-1 | This study |
HG2356 | Δfnr derived from MR-1 | (35) |
HG3099 | ΔfadL derived from MR-1 | This study |
HG3284 | ΔcydX derived from MR-1 | (59) |
HG3545 | ΔompA derived from MR-1 | This study |
HG3896 | ΔompS38 derived from MR-1 | This study |
HG3988 | ΔarcA derived from MR-1 | (44) |
HG3545-3896 | ΔompAΔompS38 (Δdouble) derived from MR-1 | This study |
Plasmid | ||
pHGM01 | Apr, Gmr, Cmr, att-based suicide vector | (33) |
pHG101 | Kmr, promoterless broad-host vector | (34) |
pHGEI01 | Kmr, integrative lacZ reporter vector | (48) |
pBBR-Cre | Spr, helper plasmid for antibiotic cassette removal | (38) |
pBXcmT | B1H bait vector | (45) |
pTRG | B1H target vector | Stratagene |
pHGE-PompS38-lacZ | pHGEI01 containing the ompS38 promoter | This study |