Table 1 Strains and plasmids used in this study.

From: Positive regulation of the Shewanella oneidensis OmpS38, a major porin facilitating anaerobic respiration, by Crp and Fur

Strain or plasmid

Description

Reference or source

E. coli strains

DH5α

Host for cloning

Lab stock

WM3064

ΔdapA, donor strain for conjugation

W. Metcalf, UIUC

XL1-Blue MRF’Kan

Recipient strain for one-hybrid system

Stratagene

S. oneidensis strains

MR-1

Wild type

ATCC 700550

HG0624

Δcrp derived from MR-1

(35)

HG1215

ΔSO1215 derived from MR-1

This study

HG1821

ΔSO1821 derived from MR-1

This study

HG1937

∆fur derived from MR-1

This study

HG2356

Δfnr derived from MR-1

(35)

HG3099

ΔfadL derived from MR-1

This study

HG3284

ΔcydX derived from MR-1

(59)

HG3545

ΔompA derived from MR-1

This study

HG3896

ΔompS38 derived from MR-1

This study

HG3988

ΔarcA derived from MR-1

(44)

HG3545-3896

ΔompAΔompS38double) derived from MR-1

This study

Plasmid

pHGM01

Apr, Gmr, Cmr, att-based suicide vector

(33)

pHG101

Kmr, promoterless broad-host vector

(34)

pHGEI01

Kmr, integrative lacZ reporter vector

(48)

pBBR-Cre

Spr, helper plasmid for antibiotic cassette removal

(38)

pBXcmT

B1H bait vector

(45)

pTRG

B1H target vector

Stratagene

pHGE-PompS38-lacZ

pHGEI01 containing the ompS38 promoter

This study