Table 2 Bacterial one-hybrid (B1H) assay of Crp with various promoters.
Bait Vector pBXcmT | Target Vector pTRG | Colonies on nonselective platesa | Colonies on selective platesb | Confirmationc |
|---|---|---|---|---|
/— | /— | 167 | 0 | — |
/— | /Crp | 176 | 0 | — |
/Pcyd | /Crp | 203 | 175 | 172 |
/Pcyd | /— | 163 | 0 | — |
/— | /Crp | 188 | 0 | — |
/P16S | /Crp | 156 | 1 | 0 |
/PompS38 | /Crp | 144 | 132 | 128 |
/PompA | /Crp | 138 | 1 | 0 |