Table 2 Bacterial one-hybrid (B1H) assay of Crp with various promoters.

From: Positive regulation of the Shewanella oneidensis OmpS38, a major porin facilitating anaerobic respiration, by Crp and Fur

Bait Vector pBXcmT

Target Vector pTRG

Colonies on nonselective platesa

Colonies on selective platesb

Confirmationc

/—

/—

167

0

/—

/Crp

176

0

/Pcyd

/Crp

203

175

172

/Pcyd

/—

163

0

/—

/Crp

188

0

/P16S

/Crp

156

1

0

/PompS38

/Crp

144

132

128

/PompA

/Crp

138

1

0

  1. aM9 agar + 25 μg/ml chloramphenicol + 12.5 μg/ml tetracycline.
  2. ba + 5 mM 3-AT.
  3. cb + 12.5 μg/ml streptomycin.