Figure 2
SETD7 silencing facilitates ROS clearance.
(a) Intracellular ROS in both scramble and SETD7 knockdown Beas-2B cells were measured by cell-permeable fluorogenic probe DCFH-DA. (b) Mitochondrial superoxide level was quantified by MitoSox in Beas-2B cell in the presence of SETD7 siRNA. (c,d) Mitochondrial superoxide accumulation was visualized and quantified in NHLFs using MitoSox live cell imaging dye (red). Nuclei were counterstained with DAPI (blue). Scale bar = 50 μM. * indicates P < 0.05. (e–h) Transcriptional levels of superoxide dismutase 2 (SOD2) and catalase (CAT) was determined by qRT-PCR in the SETD7 knockdown Beas-2B cells under CSE- or H2O2 -stimulated condition. (i) Overexpression of SETD7 in Beas-2B cells after transfection of SETD7 wild type plasmid was determined by Western blot. (j,k) SOD2 and CAT mRNAs were quantified by qRT-PCR after transfection of pSETD7 vectors and oxidative stress induction by H2O2. Data were expressed as mean ± standard error of the mean (SEM). n = 3. * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001 (two-way ANOVA).
