Figure 2

IL-37 expression in CD4⁺CD25⁺Tregs as shown by confocal laser scanning microscopy.

After stimulation with human Treg expander for 72 h, IL-37 protein expression in CD4⁺CD25⁺Tregs was determined with confocal laser scanning microscopy. IL-37 protein was shown by antibodies indirectly labelled with FITC (green) (A,B). Representative photomicrographs showed that FITC-positive cells (green) were detected among CD4⁺CD25⁺Tregs. The cells in (A) were magnified in (B). As shown in (B) it was found that IL-37 was a cytoplasmic protein and expressed abundantly in stimulated CD4⁺CD25⁺Tregs.