Figure 4
From: Enzyme-linked DNA dendrimer nanosensors for acetylcholine
(a) Largest (left) to smallest (right) schematics of the nanosensor structures with associated enzymes. (b) Initial rates of nanosensors and butyrylcholinesterase in solution with fluorescein labeled DNA dendrimers were determined in response to acetylcholine in the physiological range (Km = 1.05 mM, n = 3, black line; Km = 1.05 mM, n = 3, gray line). (c) The relative activity of nanosensor subunits and truncated nanosensors in the presence of 1 mM acetylcholine was tested. Single dendrimers, truncated dendrimers and fluorescein labeled enzyme showed a significant decrease in activity (p < 0.005, n = 3) in comparison to full nanosensors. Initial rates calculated at 30 seconds for all trials.
