Table 1 Purification of nitrite reductase from Boletus edulis fruiting bodies.

From: Boletus edulis Nitrite Reductase Reduces Nitrite Content of Pickles and Mitigates Intoxication in Nitrite-intoxicated Mice

 

Protein (mg)

Total activity (U)

Specific activity (U/mg)

Purification fold

Yield (%)

Crude extract

29250.0

22229.6

0.8

1.0

100

Ammonium sulfate precipitation (25%–65%)

4823.0

8055.7

1.7

2.2

36.2

SP-Sepharose and then followed by Q-Sepharose

414.4

3040.5

7.3

9.7

13.7

Mono Q 4.6/100 PE

84.0

977.0

11.6

15.3

4.4

Superdex75

22.1

259.4

11.8

15.5

1.2

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