Table 1 MRM validation of differentially expressed proteins associated with active immunization in R. chinensis.
Function | Protein name | Unigene ID | Peptide sequence | Fold change (Mean) | P-value |
|---|---|---|---|---|---|
Stress response proteins | 60S ribosomal protein L23 | Znev_11393 | DYDALDVANK | 1.57 | 0.01 |
| Â | isocitrate dehydrogenase | Znev_13297 | VTIIPGDGIGPEISAAVQK | 1.65 | 0.04 |
| Â | Â | Znev_13297 | SLEGYETLYDNVDVVTIR | 1.22 | 0.29 |
| Â | Â | Znev_13297 | ENTEGEYSGIEHEIVDGVVQSIK | 1.94 | 0.02 |
| Â | glutathione S-transferase D1 | Znev_15569 | LYFDIGTLYQR | 0.59 | 0.00 |
| Â | cuticle protein 19 | Znev_10404 | AAPAVDYYAYPK | 0.61 | 0.05 |
| Â | Â | Znev_10404 | GEYSLVEPDGTVR | 0.44 | 0.01 |
| Â | protein-disulfide isomerase | Znev_03262 | VLVSSNFDEVAFNK | 1.34 | 0.14 |
Immune signaling molecules | GTPase Ras | Znev_14471 | SFEDIGGYR | 2.10 | 0.06 |
| Â | ubiquitin conjugating enzyme | Znev_05594 | TDQVIQALVALVNDPEPEHPLR | 0.57 | 0.00 |
| Â | Â | Znev_05594 | ADLAEEYLK | 0.69 | 0.00 |
| Â | 26S proteasome | Znev_05722 | IVAFVGSPVETEEK | 0.94 | 0.45 |
Biosynthesis | hypothetical protein SINV_06138 | Znev_09984 | LFIGGLDYR | 0.67 | 0.00 |
| Â | Â | Znev_09984 | GFGFITYSR | 0.92 | 0.50 |
| Â | Â | Znev_09984 | GFGFVEFEDYDPVDK | 0.54 | 0.01 |
| Â | copa protein | Znev_06668 | LVGQSIIAYLQQK | 1.03 | 0.68 |
| Â | Â | Znev_06668 | LSFLYLITGNLDK | 0.52 | 0.01 |
Metabolism | transketolase-like protein 2 | Znev_11074 | SIPGSTVFYPSDAVSTER | 3.46 | 0.00 |
| Â | phosphoglycerate mutase 1 | Znev_02731 | FDIAHTSVLTR | 1.21 | 0.61 |
Development | troponin i | Znev_15251 | FDLEYAVK | 0.61 | 0.00 |
| Â | Â | Znev_15251 | DFEISDLNAQVNDLR | 0.65 | 0.02 |
| Â | PREDICTED: annexin-B9-like | Znev_05200 | EFAGSLEDGYLSIVK | 1.16 | 0.40 |
