Table 2 De novo assembly outputs of D. hirsuta transcriptome using gsAssembler (Newbler v2.5.3).

From: Transcriptome sequencing of a thalloid bryophyte; Dumortiera hirsuta (Sw) Nees: assembly, annotation and marker discovery

Reads used for assembly (excluding ribosomal and <50 bases)

425038

All Contigs (≥100 bp)

12439

Large contigs (≥500 bp)

8007

Singletons

72801

Average contig size (bp)

989

Largest contig Size (bp)

4843

N50 contig sizea (bp)

1071

% Reads Aligned

80.7

Q40 Plus basesb

95.44

  1. aN50 corresponds to the length of the smallest contig in the set of largest contigs whose combined length represents 50% of the total assembly size.
  2. bQ40Plus bases percentage of bases called that have a quality score of 40 or above.
Back to article page