Figure 3
Functional competition between ORG27569 and cholesterol.
(a) Rat brain membranes were left untreated (blue bar) or were cholesterol depleted by treatement with MβC (10 mM, 15 minutes) to be then replenished (red bar) or not (green bar) with soluble cholesterol (1 mM, 15 minutes). In each bar is indicated the amount cholesterol measured in the membranes after each treatment (expressed as % of the amount present in untreated samples, see methods for details). T1117 binding measurement performed as described in Fig.1b. Specific binding is indicated (data depict the mean +/− s.e.m. and are representative of 4 independent experiments. P < 0.05. One-way ANOVA-test was employed). (b) Rat brain membranes were treated with MβC and then replenished or not with cholesterol as described above. Membranes were incubated with the indicated amount of ORG27569. T1117 (2.5 μM) was then added and specific binding measured as described in Fig. 1b. Data were fitted with a dose response curve as described in the Method Sections. (data depict the mean +/− s.e.m. and are representative of three or more independent experiments. One-way ANOVA was employed. P < 0.05).
