Figure 4 | Scientific Reports

Figure 4

From: Endogenous vs Exogenous Allosteric Modulators in GPCRs: A dispute for shuttling CB1 among different membrane microenvironments

Figure 4

ORG27569 and cholesterol dependent shuttling of CB1 among axons and soma of the neurons.

SHSY-5Y were treated with MβC and replenished or not with cholesterol. After cholesterol manipulation cells were treated or not with ORG27569 (3 μM) for the indicated time. After being fixed and permeabilized, cells were processed for immunfluorescence to detect endogenous CB1 receptor (panel (a)), endogenous CB2 (panel (b)) or transiently expressed CB1-H2.41L-GFP (panel (b)). White arrows and red arrows indicate axonal region and soma of the cell, respectively.

Back to article page