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Figure 1

From: Trypsin- and low pH-mediated fusogenicity of avian metapneumovirus fusion proteins is determined by residues at positions 100, 101 and 294

Figure 1

Effect of trypsin on membrane fusion induced through aMPV F proteins.

(a) aMPV F proteins induced syncytium in Vero cells with or without trypsin treatment (magnification: 100×, 8 cm ×8 cm as shown). (b,c) The fusogenic activity of aMPV F proteins with or without trypsin treatment was analyzed based on syncytium formation and reporter gene assays. P < 0.05 and P < 0.005 are respectively presented as **and ***. (d) The cleavage of aMPV F proteins was analyzed using biotinylation and western blot analyses. (e) The effect of the duration of the trypsin treatment on the fusogenic activity of aMPV/C F protein. The data represent the ratio of treated vs. untreated aMPV/C F protein.

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