Figure 4
From: Discovery of Small Molecules that Inhibit the Disordered Protein, p27Kip1
Fluorescence anisotropy analysis of displacement of p27-D2-FL from Cdk2/cyclin A by SJ403.
(a) Fluorescence anisotropy analysis of Cdk2/cyclin A binding to p27-D2-FL. The starting condition (20 nM p27-D2-FL, 300 nM Cdk2/cyclin A) for assessing the effect of fragment hit SJ403 on p27 function is represented as the red circle on the binding isotherm. (b) Titration of SJ403 (0–3 mM) caused the displacement of p27-D2 from Cdk2/cyclin A, resulting in a decrease in fluorescence anisotropy values. Experiments were performed in triplicate and average values and the standard deviations of the means are shown.
